Dynamic effects of short-term exercise on muscle texture and gut flora in the hybrid (Megalobrama amblycephala♀ × Ancherythroculter nigrocauda♂)

Fillet texture plays a significant role in consumer acceptance, which heavily influenced by exercise according to previous studies. “Xianfeng No. 2”, novel hybrid of Megalobrama amblycephala ( M. ) (♀) and Ancherythroculter nigrocauda A. (♂), with characteristics quick growth, good disease resistance, anti-stress capability, is one an important aquaculture fish species China, however, the ways improve fillet quality this remains unknown. In study, short-term trial (0-16d), hematoxylin eosin (H&E) staining, analysis were carried out determine effects on muscle cellularity texture. Furthermore, 16S rRNA was performed impacts intestinal microbiota. Compared non-exercised group (0d), exercised groups showed influences (decreased fiber diameter increased density) improvements (increased hardness, cohesiveness, gumminess, chewiness). addition, sequencing exhibited that could significantly increase community richness diversity, as well alter composition gut microflora species. At level phylum, relative Proteobacteria , Firmicutes Bacteroidetes Fusobacteria changed remarkably after exercise. genus, Pseudomonas Acinetobacter Stenotrophomonas Alpinimonas Rhodococcus Shewanella Brevundimonas significantly, suggested swimming might intrinsic antimicrobial resistance promote denitrification capacity LEfSe communities illustrated MNG7 may be utilized distinguish between fish. Our research, collectively, found effective way exercise, revealed microbiota, further expanded our knowledge under